Ration to Produce Milk High in Conjugated Linoleic Acid (CLA) at Smallholder Dairy Farm: An <i>In Vitro</i> Reconstruction

Smallholder dairy farms have shown the best practices for producing milk with high Conjugated Linoleic Acid (CLA). The rations need to be further investigated explain digestion process. This study aimed reconstruct and evaluate process of five that produced CLA using in vitro method. were collected from traditional (P1, P2, P3, P4, P5) after screening 260 samples 60 at different smallholder cattle West Java Province, Indonesia. Digestibility ration was tested a two-stage result shows consist feeds nutrients. P4 better than other its simplicity, nutrient sufficiency, fermentation, digestibility, microbial synthesis. consists 40.27% Napier grass 59.72% cooperative concentrates lowest crude fiber (10.20%) highest protein (12.63%). percentage line ammonia concentration. significantly molar proportion butyrate (12.68%), total digestible (59.9%), VFA (132.23 mm), dry matter organic digestibility (77.95 74.49%), protozoa (6.31 log cell/mL) bacteria number 7.19 CFU. It is concluded 40% 60% concentrate produce milk.